The KnowPulse KnowledgeBase focuses on short question-based lessons to help researchers get their work done.
- Purpose: This lesson will show our new researchers some of the protocols we use in our research laboratory.
- Who: Pulse Crop Researchers
- Maintainer(s): Robert Stonehouse, Ruobin Liu
- Original Author(s): Robert Stonehouse, Akiko Tomita
This lesson is providing some essential laboratory protocols to guide new researchers to conduct their experiments.
Some of the things you will learn include:
How to crude DNA Isolation from seeds
How to extract DNA from fresh pulse tissues
How to use PCR program for KASP assays, then plot the results data
These protocols were originally developed by Wang H, Qi M, Cutler AJ (1993; DOI 10.1093/nar/21.17.4153) and have been optimized for use in legumes by Robert Stonehouse and Akiko Tomita in Dr. Kirstin Bett’s Molecular Pulse Crop Research lab at the University of Saskatchewan. Ruobin Liu worked with Robert Stonehouse to format these protocols for this tutorial.Stonehouse R and Tomita A. Bett, KE. 2022. Molecular marker assay from DNA extraction protocol for the USASK Pulse Molecular Biology Laboratory [lab protocol]. Saskatoon (SK): University of Saskatchewan.
The KnowPulse KnowledgeBase lessons are hands-on, so participants are encouraged to use their own computers to ensure the proper setup of tools for an efficient workflow. To most effectively use these materials, please make sure to download the data and install everything before working through this lesson.
This workshop assumes no prior experience with the tools covered in the workshop.
To get started, follow the directions in the Setup tab to get access to the required software and data for this workshop.
DNA Extraction from Pulse Crop