Searching for a Germplasm
Overview
Teaching: 5 min
Exercises: 5 minQuestions
How to search for a specific lentil cross on KnowPulse?
Objectives
Providing a step by step demonstration on how to use germplasm search on KnowPulse.
Germplasm Search—Lentil Breeding Scenario
When you land on the KnowPulse, right-click on Germplasm on the top banner, it brings you to the data section.
So simply, you go to the germplasm search and click on the image of the targeted crop, then the genus will be filled by default. Here, I am using Lens culinaris to walk you through. You are encouraged to choose the species name specifically to narrow down the results. Also, there is a category drop down to select which category the germplasm belongs to. The more information you put in, the easier it is to find the individual you are looking for.
Key Points
Log in KnowPulse with your user account before searching.
Click on the crop image before the germplasm search.
Pedigree chart view and parents search
Overview
Teaching: 5 min
Exercises: 5 minQuestions
How to view pedigree chart for a given cross?
Objectives
Providing a step by step demonstration on how to view a pedigree chart.
I am using 2545S as an example to show the pedigree chart. The legend here shows us how each selection looks like. The shaded nodes are clickable, this means there is parental data for them.
By double clicking on them, you are able to trace the full tree diagram for 2545S.
We notice 2545S has CDC Robin as its maternal parent, let us look it into details.
Key Points
Log in KnowPulse with your user account before searching.
Click on the crop image before the germplasm search.
Accessing phenotypic data for parents
Overview
Teaching: 5 min
Exercises: 5 minQuestions
How to access phenotypic data for parents?
Objectives
Providing a step by step demonstration on how to find phenotypic data for a given breeding cross.
After landing on CDC Robin’s home page, you scroll down to the bottom to see the availability of phenotypic data. When taking a precious look at the phenotypic data section, you may notice CDC Robin AGL,which is slightly different from CDC Robin.To see the values for any interesting trait, for example Plant Biomass, make sure you click on the CDC Robin AGL box, this will bring you to the germplasm page for CDC Robin AGL. On this page, a trait distribution chart can be viewed on the bottom.
Use the drop-down to select AGILE Activity 2, then select plant biomass; now a violin plot is generated automatically. The x-axis here shows the year and growing locations while the y-axis shows the single plant biomass in grams. You can see distribution of the trait across multiple site years.
Key Points
Log in KnowPulse with your user account before searching.
Click on the crop image before the germplasm search.