EVOLVES Activity 2a analyses protocols

The KnowPulse KnowledgeBase focuses on short question-based lessons to help researchers get their work done.

This lesson is providing some essential laboratory protocols to guide new researchers to conduct their experiments..

Some of the things you will learn include:

Getting Started

The KnowPulse KnowledgeBase lessons are hands-on, so participants are encouraged to use their own computers to ensure the proper setup of tools for an efficient workflow. To most effectively use these materials, please make sure to download the data and install everything before working through this lesson.

This workshop assumes no prior experience with the tools covered in the workshop.

To get started, follow the directions in the Setup tab to get access to the required software and data for this workshop.

Data

Lentil

Schedule

Setup Download files required for the lesson
00:00 1. Part 1: Preparing Standard Curves for Determining Free Sulfhydryl (SH) and Soluble Protein How to prepare standard curves for determining free sulfhydryl concentration?
How to prepare standard curves for determining soluble protein concentration?
01:00 2. Part 2: Preparing Lentil Extract, Determining Free Sulfhydryl (SH) and Soluble Protein Content How to prepare lentil extract?
How to determine free sulfhydryl concentration?
02:00 3. Protocol Diagrams How to preparing fresh lentil extract using refined method?
How to determining free sulfhydryl(SH) concentration?
How to determining soluble protein concentation?
03:00 4. Methods
03:00 Finish

The actual schedule may vary slightly depending on the topics and exercises chosen by the instructor.